Molecular immune signatures of HIV-1 vaccines in human PBMCs

Monaco, Alessandro; Marincola, Francesco M.; Sabatino, Marianna; Pos, Zoltan; Tornesello, Maria Lina; Stroncek, David F.; Wang, Ena; Lewis, George K.; Buonaguro, Franco M.; Buonaguro, Luigi

doi:10.1016/j.febslet.2009.07.060

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Volume 583, Issue 18 , Pages 3004-3008, 17 September 2009

Molecular immune signatures of HIV-1 vaccines in human PBMCs

Edited by Ivan Sadowski

Alessandro Monaco
- Infectious Disease and Immunogenetics Section (IDIS), Department of Transfusion Medicine, Clinical Center, National Institutes of Health Bethesda, MD, USA
- IRCCS Istituto Nazionale Tumori “Giovanni Paolo II”, Bari, Italy
Francesco M. Marincola
- Infectious Disease and Immunogenetics Section (IDIS), Department of Transfusion Medicine, Clinical Center, National Institutes of Health Bethesda, MD, USA
Marianna Sabatino
- Infectious Disease and Immunogenetics Section (IDIS), Department of Transfusion Medicine, Clinical Center, National Institutes of Health Bethesda, MD, USA
Zoltan Pos
- Infectious Disease and Immunogenetics Section (IDIS), Department of Transfusion Medicine, Clinical Center, National Institutes of Health Bethesda, MD, USA
Maria Lina Tornesello
- Laboratory of Molecular Biology and Viral Oncogenesis & AIDS Reference Center, Istituto Nazionale Tumori “Fondazione G. Pascale”, Naples, Italy
David F. Stroncek
- Infectious Disease and Immunogenetics Section (IDIS), Department of Transfusion Medicine, Clinical Center, National Institutes of Health Bethesda, MD, USA
Ena Wang
- Infectious Disease and Immunogenetics Section (IDIS), Department of Transfusion Medicine, Clinical Center, National Institutes of Health Bethesda, MD, USA
George K. Lewis
- Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD, USA
Franco M. Buonaguro
- Laboratory of Molecular Biology and Viral Oncogenesis & AIDS Reference Center, Istituto Nazionale Tumori “Fondazione G. Pascale”, Naples, Italy
Luigi Buonaguro
- Laboratory of Molecular Biology and Viral Oncogenesis & AIDS Reference Center, Istituto Nazionale Tumori “Fondazione G. Pascale”, Naples, Italy
- Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD, USA
- Corresponding author. Address: Laboratory of Molecular Biology and Viral Oncogenesis and AIDS Reference Center, Istituto Nazionale Tumori “Fond. G. Pascale”, Via Mariano Semmola, 1, 80131 Naples, Italy. Fax: +39 081 5451276.

Received 23 July 2009; received in revised form 30 July 2009; accepted 31 July 2009. published online 06 August 2009.

Abstract

The global transcriptional profile of peripheral blood mononuclear cells (PBMCs) stimulated with HIV candidate vaccine (virus-like particles, VLPs) has been evaluated in HIV-infected patients with low/high viral load compared to healthy volunteers. Baseline activation of chemokine production was observed in PBMC from HIV-infected patients and innate immune stimulation with HIV-VLPs was not blunted. The immune profile among HIV-infected patients was found to be qualitatively similar but quantitatively extremely variable. This diversity was independent of viral load and it might be dependent on individual immunogenetic traits or concurrent immunological status.

This ex vivo screening strategy represents an efficient tool for guiding modifications/optimizations of vaccination strategies and understanding failures in individuals enrolled in clinical trials.

Keywords: Immunogenomics, Vaccine, HIV-1, Peripheral blood mononuclear cell