Lumcorin: A leucine-rich repeat 9-derived peptide from human lumican inhibiting melanoma cell migration

Zeltz, Cédric; Brézillon, Stéphane; Perreau, Corinne; Ramont, Laurent; Maquart, François-Xavier; Wegrowski, Yanusz

doi:10.1016/j.febslet.2009.08.012

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Volume 583, Issue 18 , Pages 3027-3032, 17 September 2009

Lumcorin: A leucine-rich repeat 9-derived peptide from human lumican inhibiting melanoma cell migration

Edited by Veli-Pekka Lehto

Cédric Zeltz
- Laboratoire de Biochimie Médicale et de Biologie Moléculaire, CNRS UMR 6237, Université Reims-Champagne-Ardenne, F-51095 Reims, France
- These two authors contributed equally to this work.
Stéphane Brézillon
- Laboratoire de Biochimie Médicale et de Biologie Moléculaire, CNRS UMR 6237, Université Reims-Champagne-Ardenne, F-51095 Reims, France
- These two authors contributed equally to this work.
Corinne Perreau
- Laboratoire de Biochimie Médicale et de Biologie Moléculaire, CNRS UMR 6237, Université Reims-Champagne-Ardenne, F-51095 Reims, France
Laurent Ramont
- Laboratoire de Biochimie Médicale et de Biologie Moléculaire, CNRS UMR 6237, Université Reims-Champagne-Ardenne, F-51095 Reims, France
- CHU de Reims, F-51095 Reims, France
François-Xavier Maquart
- Laboratoire de Biochimie Médicale et de Biologie Moléculaire, CNRS UMR 6237, Université Reims-Champagne-Ardenne, F-51095 Reims, France
- CHU de Reims, F-51095 Reims, France
Yanusz Wegrowski
- Laboratoire de Biochimie Médicale et de Biologie Moléculaire, CNRS UMR 6237, Université Reims-Champagne-Ardenne, F-51095 Reims, France
- Corresponding author. Fax: +33 326 918055.

Received 11 June 2009; received in revised form 31 July 2009; accepted 7 August 2009. published online 17 August 2009.

Abstract

We previously showed that lumican decreases melanoma progression. The aim of the present study was to determine the active sequence of the lumican core protein responsible for the inhibition of melanoma cell migration. Using different recombinant and synthetic peptides derived from lumican, we localized an active site in the leucine-rich repeat 9 domain of the lumican core protein. We propose the name lumcorin (fragment of lumican core protein) for the active peptide derived from this site. Lumcorin was able to inhibit melanoma cell migration in vitro.

Abbreviations: BSA, bovine serum albumin, ECM, extracellular matrix, LRR, leucine-rich repeat, SLRP, small leucine-rich proteoglycan

Keywords: Lumican, Melanoma, Cell migration, Anti-tumor peptide, Leucine-rich repeat