Apoptosis signal-regulating kinase 1 regulates the expression of caspase-11

Choi, Jong-Ryoul; Heo, Hyejung; Lang, Yoo; Shin, Ki Soon; Kang, Shin Jung

doi:10.1016/j.febslet.2009.08.014

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Volume 583, Issue 18 , Pages 3016-3020, 17 September 2009

Apoptosis signal-regulating kinase 1 regulates the expression of caspase-11

Jong-Ryoul Choi
- Department of Molecular Biology, Sejong University, Seoul 143-747, Republic of Korea
- These authors equally contributed to this work.
Hyejung Heo
- Department of Molecular Biology, Sejong University, Seoul 143-747, Republic of Korea
- These authors equally contributed to this work.
Yoo Lang
- Department of Molecular Biology, Sejong University, Seoul 143-747, Republic of Korea
Ki Soon Shin
- Department of Biology, Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea
Shin Jung Kang
- Department of Molecular Biology, Sejong University, Seoul 143-747, Republic of Korea
- Corresponding author. Address: 98 Gunja-dong, Gwangjin-gu, Seoul 143-747, Republic of Korea. Fax: +82 2 3408 4336.

Received 8 June 2009; received in revised form 24 July 2009; accepted 11 August 2009. published online 18 August 2009.

Edited by Giulio Superti-Furga

Abstract

Caspase-11 is an inducible caspase involved in the regulation of cell death and inflammation. In the present study, we examined whether apoptosis signal-regulating kinase 1 (Ask1)-mediated signaling pathway is involved in the expression of caspase-11 induced by lipopolysaccharide (LPS). We found that the induction of caspase-11 was suppressed by the inhibitors of NADPH oxidase (Nox) or knockdown of Nox4 that acts downstream of toll-like receptor 4 and generates Ask1-activating reactive oxygen species. Overexpression of dominant negative tumor necrosis factor receptor associate factor 6 also suppressed the induction of caspase-11. Importantly, knockdown or dominant negative form of Ask1 suppressed the induction of caspase-11 following LPS stimulation. Taken together, our results show that Ask1 regulates the expression of caspase-11 following LPS stimulation.

Keywords: Ask1, Caspase-11, LPS, Nox4, TLR4