FEBS Letters
Volume 583, Issue 18 , Pages 2991-2996, 17 September 2009

DnaK-mediated association of ClpB to protein aggregates. A bichaperone network at the aggregate surface

Edited by Felix Wieland

Unidad de Biofísica (CSIC-UPV/EHU), and Departamento de Bioquímica y Biología Molecular (UPV/EHU), Facultad de Ciencia y Tecnología, Universidad del País Vasco, P.O. Box 644, Bilbao, Spain

Received 31 July 2009; received in revised form 10 August 2009; accepted 10 August 2009. published online 19 August 2009.

Abstract 

Intracellular protein aggregates formed under severe thermal stress can be reactivated by the concerted action of the Hsp70 system and Hsp100 chaperones. We analyzed here the interaction of DnaJ/DnaK and ClpB with protein aggregates. We show that aggregate properties modulate chaperone binding, which in turn determines aggregate reactivation efficiency. ClpB binding strictly depends on previous DnaK association with the aggregate. The affinity of ClpB for the aggregate-DnaK complex is low (Kd=5–10μM), indicating a weak interaction. Therefore, formation of the DnaK–ClpB bichaperone network is a three step process. After initial DnaJ binding, the cochaperone drives association of DnaK to aggregates, and in the third step, as shown here, DnaK mediates ClpB interaction with the aggregate surface.

Structured summary

MINT-7258957: G6PDH (uniprotkb:P0AC53) and G6PDH (uniprotkb:P0AC53) bind (MI:0407) by dynamic light scattering (MI:0038)MINT-7258951: alpha glucosidase (uniprotkb:P21517) and alpha glucosidase (uniprotkb:P21517) bind (MI:0407) by dynamic light scattering (MI:0038)

MINT-7258903: AdhE (uniprotkb:P0A9Q7) and AdhE (uniprotkb:P0A9Q7) bind (MI:0407) by dynamic light scattering (MI:0038)

MINT-7258900: G6PDH (uniprotkb:P0AC53) and G6PDH (uniprotkb:P0AC53) bind (MI:0407) by biophysical (MI:0013)

MINT-7258974: DnaK (uniprotkb:P0A6Y8), ClpB (uniprotkb:P63284), DnaJ (uniprotkb:P08622) and G6PDH (uniprotkb:P0AC53) physically interact (MI:0914) by cosedimentation (MI:0027)

Keywords: ClpB, DnaK, Chaperone association, Protein aggregation, Aggregate reactivation

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PII: S0014-5793(09)00637-1

doi:10.1016/j.febslet.2009.08.020

Refers to corrigendum:

  • Corrigendum to “DnaK-mediated association of ClpB to protein aggregates. A bichaperone network at the aggregate surface” [FEBS Lett. 583 (2009) 2991–2996] , 15 September 2009

    Sergio P. Acebrón, Ianire Martín, Urko del Castillo, Fernando Moro, Arturo Muga
    FEBS Letters 6 October 2009 (Vol. 583, Issue 19, Page 3301)

FEBS Letters
Volume 583, Issue 18 , Pages 2991-2996, 17 September 2009

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