FEBS Letters
Volume 583, Issue 19 , Pages 3115-3120, 6 October 2009

The Med8 mediator subunit interacts with the Rpb4 subunit of RNA polymerase II and Ace2 transcriptional activator in Schizosaccharomyces pombe

Edited by Francesc Posas

  • Surbhi Mehta

      Affiliations

    • University School of Biotechnology, G.G.S. Indraprastha University, Kashmere Gate, Delhi 110 403, India
    • ,
  • Ida Miklos

      Affiliations

    • Department of Genetics and Applied Microbiology, University of Debreccan, P.O. Box 56, Hungary
    • ,
  • Matthias Sipiczki

      Affiliations

    • Department of Genetics and Applied Microbiology, University of Debreccan, P.O. Box 56, Hungary
    • ,
  • Sagar Sengupta

      Affiliations

    • Signal Transduction Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India
    • ,
  • Nimisha Sharma

      Affiliations

    • University School of Biotechnology, G.G.S. Indraprastha University, Kashmere Gate, Delhi 110 403, India
    • Corresponding Author InformationCorresponding author. Fax: +91 11 23865941.

Received 18 August 2009; accepted 24 August 2009. published online 31 August 2009.

Abstract 

Several proteins are involved in separation of cells following division. However, their mutual interactions leading to cell separation is complex and not well understood. To explore the protein network that regulates this process at the transcriptional level in Schizosaccharomyces pombe, we have investigated the role of three proteins Med8, Rpb4 and Ace2. Using genetic and biochemical approaches we demonstrate that Ace2 binds Med8, which in turn interacts with Rpb4. We have delineated regions of Med8 and Rpb4 involved in their binding. We show that Med8 carboxyl-terminal region is necessary for its interaction with Rpb4 and can partially complement the sep15-598 mutant. Our results suggest that Med8 mediator subunit is involved in transmitting regulatory information from Ace2 to RNA polymerase II via Rpb4.

Structured summary

MINT-7260753: med8 (uniprotkb:O94646) binds (MI:0407) to ace2 (uniprotkb:O14258) by pull down (MI:0096)

MINT-7260782, MINT-7260720: rpb4 (uniprotkb:O74825) physically interacts (MI:0915) with med8 (uniprotkb:O94646) by two hybrid (MI:0018)

MINT-7260735: rpb4 (uniprotkb:O74825) binds (MI:0407) to med8 (uniprotkb:O94646) by pull down (MI:0096)

Abbreviations: pol II, RNA polymerase II, RT, reverse transcriptase

Keywords: Rpb4, Med8, Sep15, Ace2

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PII: S0014-5793(09)00669-3

doi:10.1016/j.febslet.2009.08.036

FEBS Letters
Volume 583, Issue 19 , Pages 3115-3120, 6 October 2009

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