Identification of amino acid residues of influenza A virus H3 HA contributing to the recognition of molecular species of sialic acid

Takahashi, Tadanobu; Hashimoto, Asako; Maruyama, Mami; Ishida, Hideharu; Kiso, Makoto; Kawaoka, Yoshihiro; Suzuki, Yasuo; Suzuki, Takashi

doi:10.1016/j.febslet.2009.08.037

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Volume 583, Issue 19 , Pages 3171-3174, 6 October 2009

Identification of amino acid residues of influenza A virus H3 HA contributing to the recognition of molecular species of sialic acid

Edited by Hans-Dieter Klenk

Tadanobu Takahashi
- Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Shizuoka 422-8526, Japan
- CREST, Japan Science and Technology Agency, Saitama 332-0012, Japan
- Global COE Program for Innovation in Human Health Sciences, Shizuoka 422-8526, Japan
Asako Hashimoto
- Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Shizuoka 422-8526, Japan
- CREST, Japan Science and Technology Agency, Saitama 332-0012, Japan
Mami Maruyama
- Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Shizuoka 422-8526, Japan
Hideharu Ishida
- CREST, Japan Science and Technology Agency, Saitama 332-0012, Japan
- Department of Applied Bioorganic Chemistry, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan
Makoto Kiso
- CREST, Japan Science and Technology Agency, Saitama 332-0012, Japan
- Department of Applied Bioorganic Chemistry, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan
Yoshihiro Kawaoka
- CREST, Japan Science and Technology Agency, Saitama 332-0012, Japan
- International Research Centre for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
- Department of Pathobiological Sciences, University of Wisconsin, Madison, WI, USA
Yasuo Suzuki
- CREST, Japan Science and Technology Agency, Saitama 332-0012, Japan
- Global COE Program for Innovation in Human Health Sciences, Shizuoka 422-8526, Japan
- Health Science Hills, and Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Aichi 487-8501, Japan
Takashi Suzuki
- Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Shizuoka 422-8526, Japan
- CREST, Japan Science and Technology Agency, Saitama 332-0012, Japan
- Global COE Program for Innovation in Human Health Sciences, Shizuoka 422-8526, Japan
- Corresponding author. Address: Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Shizuoka 422-8526, Japan. Fax: +81 54 264 5725.

Received 16 June 2009; received in revised form 24 July 2009; accepted 26 August 2009. published online 31 August 2009.

Abstract

To identify a determinant of human H3 hemagglutinin (HA) amino acid residues linked to the recognition of molecular species of sialic acid, we generated six mutant viruses possessing either the wild-type HA gene from A/Memphis/1/71 (H3N2) or a genetically single-mutated HA gene at position 137, 144, 155, 158 or 193 from a genetic backbone of A/WSN/33 (H1N1) by reverse genetics. We evaluated the binding ability with four types of synthetic sialylglycolipids. The results indicate that the amino acid substitutions Thr155 to Tyr and Glu158 to Gly in H3 HA facilitate virus binding to N-glycolylneuraminic acid.

Abbreviations: HA, hemagglutinin, HAU, hemagglutination unit, IAV, influenza A virus, Neu5Ac, 5-N-acetylneuraminic acid, Neu5Gc, 5-N-glycolylneuraminic acid, TLC, thin-layer chromatography