p24 proteins play a role in peroxisome proliferation in yeast

Abstract 

Emp24 is a member of the p24 protein family, which was initially localized to the endoplasmic reticulum, Golgi and COP vesicles, but has recently shown to be associated with Saccharomyces cerevisiae peroxisomes as well. Using cell fractionation and electron- and fluorescence microscopy, we show that in the yeast Hansenula polymorpha, Emp24 also associates with peroxisomes. In addition, we show that peroxisome numbers are strongly decreased in H. polymorpha cells lacking two proteins of the p24 complex, Emp24 and Erp3. Detailed fluorescence microscopy analyses suggest that emp24.erp3 cells are disturbed in peroxisome fission and inheritance.

Abbreviations: AMO, amine oxidase, AOX, alcohol oxidase, ER, endoplasmic reticulum, GFP, green fluorescent protein, -SKL, C-terminal tripeptide serine–lysine–leucine, WT, wild type

Keywords: Organelle proliferation, Endoplasmic reticulum, Peroxisome, Organelle fission