FEBS Letters
Volume 583, Issue 19 , Pages 3127-3132, 6 October 2009

C-terminal binding proteins (CtBPs) attenuate KLF4-mediated transcriptional activation

Edited by Ivan Sadowski

  • Gang Liu
    • ,
  • Hai Zheng
    • ,
  • Walden Ai

      Affiliations

    • Corresponding Author InformationCorresponding author. Address: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, 6439 Garners Ferry Road, Columbia, SC 29208, United States. Fax: +1 803 733 1515.

Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC 29208, United States

Received 29 June 2009; received in revised form 22 August 2009; accepted 4 September 2009. published online 14 September 2009.

Abstract 

We aimed to examine the physical interaction between CtBPs and KLF4 and the potential importance of this interaction. Co-immunoprecipitation indicated that CtBP1 indeed interacted with KLF4. This was supported by the co-localization of both KLF4 and CtBP1 in the promoter regions of KLF4 downstream target genes. In addition, overexpression of CtBP1 significantly decreased KLF4-mediated transcriptional activation in both an artificial (pGL5) and genuine (IAP and Keratin-4) reporter system. Mutations in the potential CtBP binding motif in KLF4 were accompanied by loss of the inhibitory effect of CtBP1 in the reporter assay and of the physical interaction with CtBP1. Overall, our results suggest that CtBPs attenuate KLF4-mediated transcriptional activation through the physical interaction with KLF4.

Structured summary

MINT-7261981, MINT-7261995: KLF4 (uniprotkb:O43474) physically interacts (MI:0915) with CTBP1 (uniprotkb:Q13363) by anti tag coimmunoprecipitation (MI:0007)

MINT-7262008, MINT-7262023: CTBP1 (uniprotkb:Q13363) physically interacts (MI:0915) with KLF4 (uniprotkb:O43474) by anti bait coimmunoprecipitation (MI:0006)

Keywords: CtBP, KLF4, Transcription, Co-immunoprecipitation, Chromatin immunoprecipitation, Transient co-transfection

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PII: S0014-5793(09)00701-7

doi:10.1016/j.febslet.2009.09.013

FEBS Letters
Volume 583, Issue 19 , Pages 3127-3132, 6 October 2009

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