FEBS Letters
Volume 583, Issue 19 , Pages 3145-3150, 6 October 2009

Involvement of SigT and RstA in the differentiation of Streptomyces coelicolor

Edited by Renee Tsolis

Zhejiang University, College of Life Sciences, Hangzhou 310058, China

Received 5 August 2009; received in revised form 28 August 2009; accepted 9 September 2009. published online 14 September 2009.

Abstract 

SigT is an ECF sigma factor in Streptomyces coelicolor. sigT and its putative anti-sigma factor gene rstA are located in one putative operon, and SigT could physically interact with RstA. Deletion of sigT or rstA caused accelerated morphological development and enhanced production of antibiotics, concomitant with over-expression of chpE, chpH, actII-orf4 and redD. Furthermore, SigT was undetectable after loss of rstA. These data suggested that SigT has a negative role on differentiation and that RstA negatively regulates the SigT activity through a putative antagonistic mechanism and at the post-transcriptional level.

Structured summary

MINT-7262599, MINT-7262614: RstA (uniprotkb:Q9S6U2) physically interacts (MI:0915) with sigT (uniprotkb:O86856) by pull down (MI:0096)

MINT-7262539: rpoC (uniprotkb:Q8CJT1) physically interacts (MI:0915) with sigT (uniprotkb:O86856) by pull down (MI:0096)

MINT-7262574: RstA (uniprotkb:Q9S6U2) physically interacts (MI:0915) with sigT (uniprotkb:O86856) by anti tag coimmunoprecipitation (MI:0007)

Keywords: SigT, RstA, Differentiation, Streptomyces coelicolor

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PII: S0014-5793(09)00713-3

doi:10.1016/j.febslet.2009.09.025

FEBS Letters
Volume 583, Issue 19 , Pages 3145-3150, 6 October 2009

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