Ethylene and cold participate in the regulation of LeCBF1 gene expression in postharvest tomato fruits

Zhao, Danying; Shen, Lin; Fan, Bei; Yu, Mengmeng; Zheng, Yang; Lv, Shengnan; Sheng, Jiping

doi:10.1016/j.febslet.2009.09.029

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Volume 583, Issue 20 , Pages 3329-3334, 20 October 2009

Ethylene and cold participate in the regulation of LeCBF1 gene expression in postharvest tomato fruits

Edited by Ulf-Ingo Flügge

Danying Zhao
- College of Food Science and Nutritional Engineering, China Agriculture University, Beijing 100083, China
- Beijing Xicheng District Disease Control and Prevention Center, Beijing 100011, China
- These authors contributed to this work equally.
Lin Shen
- College of Food Science and Nutritional Engineering, China Agriculture University, Beijing 100083, China
- Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China
- These authors contributed to this work equally.
Bei Fan
- Institute of Agro-Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Mengmeng Yu
- College of Food Science and Nutritional Engineering, China Agriculture University, Beijing 100083, China
Yang Zheng
- College of Food Science and Nutritional Engineering, China Agriculture University, Beijing 100083, China
Shengnan Lv
- College of Food Science and Nutritional Engineering, China Agriculture University, Beijing 100083, China
Jiping Sheng
- College of Food Science and Nutritional Engineering, China Agriculture University, Beijing 100083, China
- Corresponding author. Fax: +86 10 62737620.

Received 6 July 2009; received in revised form 3 September 2009; accepted 3 September 2009. published online 18 September 2009.

Abstract

C-Repeat/dehydration-responsive element binding factor (CBF) is a transcription factor regulating cold response in plants, of which little is known in fruits. We showed a double-peak expression pattern of Lycopersicon esculentum putative transcriptional activator CBF1 (LeCBF1) in mature green fruit. The peaks appeared at 2 and 16h after subjection to cold storage (2°C). The second peak was coincident with, and thus caused by a peak in endogenous ethylene production. We showed that LeCBF1 expression was regulated by exogenous ethylene and 1-methylcyclopropene, and was not expressed without cold induction. LeCBF1 expression was different in the five maturation stages of fruits, but expression peaked at 2h at all stages.