FEBS Letters
Volume 583, Issue 20 , Pages 3339-3343, 20 October 2009

Multiple proteins and phosphorylations regulate Saccharomyces cerevisiae Cdc24p localization

Edited by Lukas Huber

Department of Microbiology and Molecular Genetics, University of Vermont, 202 Stafford Hall, 95 Carrigan Drive, VT 05405, United States

Received 17 August 2009; received in revised form 16 September 2009; accepted 19 September 2009. published online 24 September 2009.

Abstract 

Targeting of Saccharomyces cerevisiae Cdc24p to polarized growth sites is essential for its function. Localization of GFP-tagged Cdc24 proteins or fragments was assayed in deletion mutants of Cdc24p-interacting proteins. The boi2Δ, ent2Δ, and hua1Δ mutants showed localization defects. The tos2Δ skg6Δ double mutant displayed aberrant pre-anaphase localization to the mother-bud neck region. The same aberrant pattern was seen when potential phosphorylation sites Ser697, Thr704, and Tyr200 were mutated. The S697A mutation also resulted in phosphorylation defects in vivo. These data support roles for Boi2p, Ent2p, Hua1p, Tos2p, and for Cdc24p phosphorylation in targeting Cdc24p to growth sites.

Keywords: Cdc24p, Membrane targeting, Cell polarity, Cell-cycle phosphorylation

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PII: S0014-5793(09)00733-9

doi:10.1016/j.febslet.2009.09.033

FEBS Letters
Volume 583, Issue 20 , Pages 3339-3343, 20 October 2009

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