A scanning peptide array approach uncovers association sites within the JNK/βarrestin signalling complex

Li, Xiang; MacLeod, Ruth; Dunlop, Allan J.; Edwards, Helen V.; Advant, Noopur; Gibson, Lucien C.D.; Devine, Nicola M.; Brown, Kim M.; Adams, David R.; Houslay, Miles D.; Baillie, George S.

doi:10.1016/j.febslet.2009.09.035

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Volume 583, Issue 20 , Pages 3310-3316, 20 October 2009

A scanning peptide array approach uncovers association sites within the JNK/βarrestin signalling complex

Edited by Lukas Huber

Xiang Li
- Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Building, Glasgow University, Glasgow G12 8QQ, United Kingdom
Ruth MacLeod
- Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Building, Glasgow University, Glasgow G12 8QQ, United Kingdom
Allan J. Dunlop
- Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Building, Glasgow University, Glasgow G12 8QQ, United Kingdom
Helen V. Edwards
- Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Building, Glasgow University, Glasgow G12 8QQ, United Kingdom
Noopur Advant
- Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Building, Glasgow University, Glasgow G12 8QQ, United Kingdom
Lucien C.D. Gibson
- Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Building, Glasgow University, Glasgow G12 8QQ, United Kingdom
Nicola M. Devine
- Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Building, Glasgow University, Glasgow G12 8QQ, United Kingdom
Kim M. Brown
- Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Building, Glasgow University, Glasgow G12 8QQ, United Kingdom
David R. Adams
- Department of Chemistry, Heriot-Watt University, Riccarton Campus, Edinburgh EH14 4AS, Scotland, United Kingdom
Miles D. Houslay
- Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Building, Glasgow University, Glasgow G12 8QQ, United Kingdom
George S. Baillie
- Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Building, Glasgow University, Glasgow G12 8QQ, United Kingdom
- Corresponding author. Fax: +44 141 330 4365.

Received 9 July 2009; received in revised form 1 September 2009; accepted 16 September 2009. published online 24 September 2009.

Abstract

βarrestins are molecular scaffolds that can bring together three-component mitogen-activated protein kinase signalling modules to promote signal compartmentalisation. We use peptide array technology to define novel interfaces between components within the c-Jun N-terminal kinase (JNK)/βarrestin signalling complex. We show that βarrestin 1 and βarrestin 2 associate with JNK3 via the kinase N-terminal domain in a region that, surprisingly, does not harbour a known ‘common docking’ motif. In the N-domain and C-terminus of βarrestin 1 and βarrestin 2 we identify two novel apoptosis signal-regulating kinase 1 binding sites and in the N-domain of the βarrestin 1 and βarrestin 2 we identify a novel MKK4 docking site.

Structured summary

MINT-7263196, MINT-7263175: Arrestin beta-2 (uniprotkb:P32121) binds (MI:0407) to ASK1 (uniprotkb:Q99683) by peptide array (MI:0081)

MINT-7263136: JNK3 (uniprotkb:P53779) binds (MI:0407) to Arrestin beta-1 (uniprotkb:P49407) by peptide array (MI:0081)

MINT-7263161: JNK3 (uniprotkb:P53779) binds (MI:0407) to Arrestin beta-2 (uniprotkb:P32121) by peptide array (MI:0081)

MINT-7263304: Arrestin beta-1 (uniprotkb:P49407) physically interacts (MI:0915) with ASK1 (uniprotkb:Q99683) by anti tag coimmunoprecipitation (MI:0007)

MINT-7263286: Arrestin beta-2 (uniprotkb:P32121) binds (MI:0407) to MKK4 (uniprotkb:P45985) by peptide array (MI:0081)

MINT-7263231, MINT-7263254: Arrestin beta-1 (uniprotkb:P49407) binds (MI:0407) to ASK1 (uniprotkb:Q99683) by peptide array (MI:0081)

MINT-7263269: Arrestin beta-1 (uniprotkb:P49407) binds (MI:0407) to MKK4 (uniprotkb:P45985) by peptide array (MI:0081)

Abbreviations: MAPK, Map kinase, JNK, c-Jun N-terminal kinase, ASK1, apoptosis signal-regulating kinase 1, MKK4, MAPK kinase 4, CD site, common docking site