FEBS Letters
Volume 583, Issue 20 , Pages 3335-3338, 20 October 2009

A regulatory domain spanning the repeat sequence RE1 from herpes simplex virus type 1 has cell specific differential functions in trigeminal neurons and fibroblasts

Edited by Hans-Dieter Klenk

  • Hannah C. Stevens

      Affiliations

    • School of Biomedical Sciences, Divisions of Physiology and HACB, University of Liverpool, L69 3BX, UK
    • ,
  • Carolyn Fiskerstrand

      Affiliations

    • The Roslin Institute and R(D)SVS, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK
    • ,
  • Vivien J. Bubb

      Affiliations

    • School of Biomedical Sciences, Divisions of Physiology and HACB, University of Liverpool, L69 3BX, UK
    • ,
  • Robert Dalziel

      Affiliations

    • The Roslin Institute and R(D)SVS, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK
    • ,
  • John P. Quinn

      Affiliations

    • School of Biomedical Sciences, Divisions of Physiology and HACB, University of Liverpool, L69 3BX, UK
    • Corresponding Author InformationCorresponding author. Fax: +44 (0) 151 794 5517.

Received 17 June 2009; received in revised form 20 September 2009; accepted 23 September 2009. published online 29 September 2009.

Abstract 

In this report we demonstrate that the herpes simplex virus type 1 reiteration element 1 (RE1) (nt: 117158–117353) in concert with its flanking sequences is both a cell specific and stimulus inducible regulatory domain. This region of the virus genome and specifically the RE1 supports differential reporter gene expression in both baby hamster kidney cells and disassociated rat trigeminal ganglia and is present within a region that is implicated in regulating latency of the virus in neuronal cells. Further we demonstrate that this locus is a transcriptional regulatory domain and a target for the transcription factor CCCTC binding protein.

Abbreviations: VNTR, variable number tandem repeat, HSV-1, herpes simplex virus type 1, TAC1, preprotachykinin-A, TG, trigeminal ganglia, RE1, reiteration element 1, CTCF, CCCTC binding protein, DRG, dorsal root ganglia, CTRL1, CTCF tandem repeat long 1, CTRL2, CTCF tandem repeat long 2, SLC6A3, dopamine transporter gene, SLC6A4, serotonin transporter gene, BHK, baby hamster kidney, BGP-1, beta globin protein 1

Keywords: Herpes simplex virus type 1, Cell specific enhancer, Tandem repeat, CCCTC binding protein

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PII: S0014-5793(09)00737-6

doi:10.1016/j.febslet.2009.09.037

FEBS Letters
Volume 583, Issue 20 , Pages 3335-3338, 20 October 2009

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