Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon

Martin, Victoria; Villarreal, Fernando; Miras, Isabelle; Navaza, Alda; Haouz, Ahmed; González-Lebrero, Rodolfo M.; Kaufman, Sergio B.; Zabaleta, Eduardo

doi:10.1016/j.febslet.2009.09.055

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Volume 583, Issue 21 , Pages 3425-3430, 3 November 2009

Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon

Edited by Miguel De la Rosa

Victoria Martin
- Instituto de Investigaciones Biológicas (IIB-CONICET-UNMdP), CC 1245, 7600 Mar del Plata, Argentina
Fernando Villarreal
- Instituto de Investigaciones Biológicas (IIB-CONICET-UNMdP), CC 1245, 7600 Mar del Plata, Argentina
Isabelle Miras
- Institut Pasteur, Plate forme 6, URA CNRS 2185, 25, rue du Dr ROUX, Paris 75724, France
Alda Navaza
- ANBioPhi, FRE3207 CNRS-Université Paris6-Université Paris13, 74, rue Marcel Cachin, 93017 Bobigny, France
Ahmed Haouz
- Institut Pasteur, Plate forme 6, URA CNRS 2185, 25, rue du Dr ROUX, Paris 75724, France
Rodolfo M. González-Lebrero
- IQUIFIB-Dpto. Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
Sergio B. Kaufman
- IQUIFIB-Dpto. Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
Eduardo Zabaleta
- Instituto de Investigaciones Biológicas (IIB-CONICET-UNMdP), CC 1245, 7600 Mar del Plata, Argentina
- Corresponding author. Fax: +54 223 475 31 50.

Received 29 July 2009; received in revised form 18 September 2009; accepted 28 September 2009. published online 05 October 2009.

Abstract

Gamma carbonic anhydrases (γCA) are widespread in Prokaryotes. In Eukaryotes, homologous genes were found only in plant genomes. In Arabidopsis and maize, the corresponding gene products are subunits of mitochondrial Complex I.

At present, only γCA homotrimers of Methanosarcina thermophila (CAM) show reversible carbon dioxide (CO₂) hydration activity. In the present work, it is shown that recombinant plant γCA2 could form homotrimers and bind . However, they are unable to catalyse the reversible hydration of CO₂.

These results suggest that plant γCAs do not act as carbonic anhydrases but with a related activity possibly contributing to recycle CO₂ in the context of photorespiration.

Structured summary

MINT-7266044: gamma CA2 (uniprotkb:Q9C6B3) and gamma CA2 (uniprotkb:Q9C6B3) physically interact (MI:0914) by dynamic light scattering (MI:0038)

MINT-7266036: gamma CA2 (uniprotkb:Q9C6B3) and gamma CA2 (uniprotkb:Q9C6B3) physically interact (MI:0914) by molecular sieving (MI:0071)

Keywords: Gamma carbonic anhydrase, Carbon dioxide, Carbonate, Mitochondria, Arabidopsis thaliana