FEBS Letters
Volume 583, Issue 21 , Pages 3431-3436, 3 November 2009

The interaction between EAP30 and ELL is modulated by MCM2

Edited by Ivan Sadowski

Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1

Received 24 April 2009; received in revised form 17 September 2009; accepted 2 October 2009. published online 09 October 2009.

Abstract 

ELL-associated protein 30 (EAP30) was initially characterized as a component of the Holo–ELL complex, which contains the elongation factor ELL. Both ELL and Holo–ELL stimulate RNA pol II elongation in vitro. However, ELL and not Holo–ELL inhibits RNA pol II initiation. It is not clear how these two discrete functions of ELL are regulated. Here we report that mini-chromosome maintenance 2 (MCM2) binds to EAP30 and show that MCM2 competes with ELL for binding to EAP30 thus potentially modulating the stability of Holo–ELL.

Structured summary

MINT-7277033: EAP30 (uniprotkb:Q96H20) physically interacts (MI:0915) with RPB1 (uniprotkb:P24928) by anti tag coimmunoprecipitation (MI:0007)

MINT-7277085: EAP30 (uniprotkb:Q96H20) binds (MI:0407) to ELL (uniprotkb:P55199) by pull down (MI:0096)

MINT-7277072: EAP30 (uniprotkb:Q96H20) physically interacts (MI:0915) with ELL (uniprotkb:P55199) by anti tag coimmunoprecipitation (MI:0007)

MINT-7277100: EAP30 (uniprotkb:Q96H20) physically interacts (MI:0915) with ELL (uniprotkb:P55199) by competition binding (MI:0405)

MINT-7277153: MCM2 (uniprotkb:P49736) binds (MI:0407) to ELL (uniprotkb:P55199) by pull down (MI:0096)

MINT-7276989: EAP30 (uniprotkb:Q96H20) physically interacts (MI:0915) with MCM2 (uniprotkb:P49736) by pull down (MI:0096)

MINT-7277005: EAP30 (uniprotkb:Q96H20) physically interacts (MI:0915) with RPB1 (uniprotkb:P24928) by pull down (MI:0096)

MINT-7276960, MINT-7277168: MCM2 (uniprotkb:P49736) physically interacts (MI:0915) with EAP30 (uniprotkb:Q96H20) by two hybrid (MI:0018)

MINT-7276971, MINT-7277121, MINT-7277137: MCM2 (uniprotkb:P49736) binds (MI:0407) to EAP30 (uniprotkb:Q96H20) by pull down (MI:0096)

MINT-7277018, MINT-7277061: EAP30 (uniprotkb:Q96H20) physically interacts (MI:0915) with MCM2 (uniprotkb:P49736) by anti tag coimmunoprecipitation (MI:0007)

Abbreviations: ELL, Eleven–nineteen lysine-rich leukemia, EAP30, ELL-associated protein 30, MCM2, mini-chromosome maintenance 2, ChB, chromatography buffer, PAGE–SDS gels, polyacrylamide–sodium dodecyl sulfate gels

Keywords: Eleven–nineteen lysine-rich leukemia, ELL-associated protein 30, Mini-chromosome maintenance 2

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PII: S0014-5793(09)00780-7

doi:10.1016/j.febslet.2009.10.009

FEBS Letters
Volume 583, Issue 21 , Pages 3431-3436, 3 November 2009

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