Radioprotection by short-term oxidative preconditioning: Role of manganese superoxide dismutase

Belikova, Natalia A.; Glumac, Ashley; Rafikov, Ruslan; Jiang, Jianfei; Greenberger, Joel S.; Kagan, Valerian E.; Bayir, Hülya

doi:10.1016/j.febslet.2009.10.013

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Volume 583, Issue 21 , Pages 3437-3442, 3 November 2009

Radioprotection by short-term oxidative preconditioning: Role of manganese superoxide dismutase

Edited by Vladimir Skulachev

Natalia A. Belikova
- Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219, USA
- Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA
- Equal contribution.
Ashley Glumac
- Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219, USA
- Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA
- Equal contribution.
Ruslan Rafikov
- Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219, USA
- Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA
Jianfei Jiang
- Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219, USA
- Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA
Joel S. Greenberger
- Department of Radiation Oncology, University of Pittsburgh, Pittsburgh, PA 15260, USA
Valerian E. Kagan
- Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219, USA
- Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA
Hülya Bayir
- Department of Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA 15201, USA
- Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA
- Corresponding author. Address: Department of Critical Care Medicine, University of Pittsburgh, 45th St. & Penn Ave., Pittsburgh, PA 15201, USA. Fax: +1 412 692 6076 (H. Bayir).

Received 12 August 2009; received in revised form 3 September 2009; accepted 6 October 2009. published online 12 October 2009.

Abstract

Manganese superoxide dismutase (MnSOD) is vital to the protection of mitochondria and cells against oxidative stress. Earlier, we demonstrated that catalytically active homo-tetramer of MnSOD can be stabilized by oxidative cross-linking. Here we report that this effect may be translated into increased radioresistance of mouse embryonic cells (MECs) by pre-exposure to oxidative stress. Pre-treatment of MECs with antimycin A, rotenone or H₂O₂ increased their survival after irradiation. Using MnSOD siRNA, we show that MECs with decreased MnSOD levels displayed a lowered ability to preconditioning. Thus oxidative preconditioning may be used for targeted regulation of MnSOD.