FEBS Letters
Volume 583, Issue 24 , Pages 3966-3973, 17 December 2009

Correlation of mRNA and protein in complex biological samples

Edited by Stefan Hohmann

Center for Genomic Regulation, Systems Biology Unit, Dr. Aiguader 88, 08003 Barcelona, Spain

Received 31 August 2009; received in revised form 9 October 2009; accepted 14 October 2009. published online 20 October 2009.

Abstract 

The correlation between mRNA and protein abundances in the cell has been reported to be notoriously poor. Recent technological advances in the quantitative analysis of mRNA and protein species in complex samples allow the detailed analysis of this pathway at the center of biological systems. We give an overview of available methods for the identification and quantification of free and ribosome-bound mRNA, protein abundances and individual protein turnover rates. We review available literature on the correlation of mRNA and protein abundances and discuss biological and technical parameters influencing the correlation of these central biological molecules.

Keywords: mRNA, Protein, Quantitative, Systems biology, Genomics, Proteomics

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PII: S0014-5793(09)00812-6

doi:10.1016/j.febslet.2009.10.036

FEBS Letters
Volume 583, Issue 24 , Pages 3966-3973, 17 December 2009

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