FEBS Letters
Volume 583, Issue 22 , Pages 3549-3556, 19 November 2009

Nucleolar disruption impairs Kaposi’s sarcoma-associated herpesvirus ORF57-mediated nuclear export of intronless viral mRNAs

Edited by Hans-Dieter Klenk

  • James R. Boyne

      Affiliations

    • Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK
    • Division of Biomedical Sciences, School of Life Sciences, University of Bradford, Bradford, BD7 1DP, UK
    • ,
  • Adrian Whitehouse

      Affiliations

    • Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK
    • Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK
    • Corresponding Author InformationCorresponding author. Address: Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK. Fax: +44 (0)113 3433167.

Received 20 July 2009; received in revised form 25 September 2009; accepted 12 October 2009. published online 20 October 2009.

Abstract 

Kaposi’s sarcoma-associated herpesvirus (KSHV) ORF57 plays a pivotal role in mediating the nuclear export of KSHV intronless transcripts. We demonstrate that ORF57 localises and dynamically trafficks through the nucleolus. To assess if nucleolar shuttling of ORF57 is important for the export of intronless viral mRNA, the ability of ORF57 to export mRNA was assessed in cells where the nucleolus was disrupted using Actinomycin D or DRB. We show that rapid disorganisation of the nucleolus was accompanied by a reduction in intronless virus mRNA export, suggesting that an intact nucleolus is essential for efficient KSHV ORF57-mediated intronless mRNA export.

Structured summary

MINT-7291377: Aly (uniprotkb:Q86V81) physically interacts (MI:0915) with ORF57 (uniprotkb:Q2HR75) by pull down (MI:0096)

MINT-7291330, MINT-7291320: Nucleolin (uniprotkb:P19338) and ORF57 (uniprotkb:Q2HR75) colocalise (MI:0403) by fluorescence microscopy (MI:0416)

MINT-7291715: ORF57 (uniprotkb:Q2HR75) and Aly (uniprotkb:Q86V81) colocalise (MI:0403) by fluorescence microscopy (MI:0416)

Keywords: Nucleolus, mRNA, Nuclear export, Herpesvirus

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PII: S0014-5793(09)00824-2

doi:10.1016/j.febslet.2009.10.040

FEBS Letters
Volume 583, Issue 22 , Pages 3549-3556, 19 November 2009

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