FEBS Letters
Volume 584, Issue 1 , Pages 86-92, 4 January 2010

Shrinking and development of lipid droplets in adipocytes during catecholamine-induced lipolysis

Edited by Laszlo Nagy

  • Masafumi Nagayama

      Affiliations

    • Division of Applied Physics, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, Japan
    • Corresponding Author InformationCorresponding author. Address: Division of Applied Physics, Graduate School of Engineering, Hokkaido University, Kita 13, Nishi 8, Kita-ku, Sapporo 060-8628, Japan. Fax: +81 11 706 6637.
    • ,
  • Kyoko Shimizu

      Affiliations

    • Primary Cell Co. Ltd., Sapporo 001-0021, Japan
    • ,
  • Toshio Taira

      Affiliations

    • Primary Cell Co. Ltd., Sapporo 001-0021, Japan
    • ,
  • Tsutomu Uchida

      Affiliations

    • Division of Applied Physics, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, Japan
    • ,
  • Kazutoshi Gohara

      Affiliations

    • Division of Applied Physics, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, Japan

Received 6 August 2009; received in revised form 30 September 2009; accepted 31 October 2009. published online 06 November 2009.

Abstract 

Time-lapse observation of adipocytes during catecholamine-induced lipolysis clearly shows that shrinking of existing lipid droplets (LDs) occurs in some adipocytes and that small LDs are newly developed in almost all cells. Immunofluorescence imaging reveals that activation and localization of hormone-sensitive lipase (HSL) on the surface of LDs, which are required for conferring maximal lipolysis, are necessary for the shrinking of the LDs. However, not all adipocytes in which phosphorylated HSL is localized on LDs exhibit shrinking of LDs. The simultaneous shrinking and development of LDs yield apparent fragmentation and dispersion of LDs in adipocytes stimulated with catecholamine.

Abbreviations: ATGL, adipose triglyceride lipase, BSA, bovine serum albumin, DIC, differential interference contrast, FFA, free fatty acid, HSL, hormone-sensitive lipase, LD, lipid droplet, PBS, phosphate-buffered saline, PKA, protein kinase A, SVC, stromal-vascular cell, TG, triglyceride

Keywords: Adipocyte, Lipid droplet, Norepinephrine, Adipose triglyceride lipase, Hormone-sensitive lipase, Time-lapse observation

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PII: S0014-5793(09)00892-8

doi:10.1016/j.febslet.2009.10.088

FEBS Letters
Volume 584, Issue 1 , Pages 86-92, 4 January 2010

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