FEBS Letters
Volume 584, Issue 1 , Pages 27-32, 4 January 2010

Involvement of Src tyrosine kinase in Escherichia coli invasion of human brain microvascular endothelial cells

Edited by Renee Tsolis

Department of Developmental Biology, Key Laboratory of Cell Biology, Ministry of Public Health, 92 Beier Road, Heping Distric, Shenyang 110001, China

Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, 92 Beier Road, Heping Distric, Shenyang 110001, China

Received 6 September 2009; received in revised form 24 October 2009; accepted 30 October 2009. published online 09 November 2009.

Abstract 

Invasion of brain microvascular endothelial cells is a prerequisite for successful crossing of the blood–brain barrier by Escherichia coli (E. coli), but the underlying mechanism remains unclear. Here we showed activation of Src tyrosine kinase in E. coli K1 invasion of human brain microvascular endothelial cells (HBMEC). E. coli invasion of HBMEC and the E. coli-induced rearrangement of actin filaments were blocked by Src inhibitors. Overexpression of dominant-negative Src in HBMEC significantly attenuated E. coli invasion and the concomitant actin filaments rearrangement. Furthermore, E. coli K1-triggered phosphatidylinositol 3-kinase (PI3K) activation in HBMEC was effectively blocked by Src inhibitors and dominant-negative Src. These results demonstrated the involvement of Src and its interaction with PI3K in E. coli K1 invasion of HBMEC.

Structured summary

MINT-7296127, MINT-7296136: Src (uniprotkb:P12931) physically interacts (MI:0915) with p85 (uniprotkb:P27986) by anti bait coimmunoprecipitation (MI:0006)

MINT-7296149: F-actin (uniprotkb:P60709) and Src-DN (uniprotkb:P12931) colocalize (MI:0403) by fluorescence microscopy (MI:0416)

Keywords: Src, Escherichia coli K1, Human brain microvascular endothelial cell, Phosphatidylinositol 3-kinase, Blood–brain barrier

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PII: S0014-5793(09)00902-8

doi:10.1016/j.febslet.2009.10.090

FEBS Letters
Volume 584, Issue 1 , Pages 27-32, 4 January 2010

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