FEBS Letters
Volume 584, Issue 3 , Pages 511-516, 5 February 2010

Molecular characterization of Hydra acetylcholinesterase and its catalytic activity

Edited by Michael R. Bubb

  • Toshio Takahashi

      Affiliations

    • Suntory Institute for Bioorganic Research, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan
    • Corresponding Author InformationCorresponding author. Fax: +81 75 962 2115.
    • ,
  • Naoya Hamaue

      Affiliations

    • Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan

Received 21 August 2009; received in revised form 4 November 2009; accepted 10 November 2009. published online 30 November 2009.

Abstract 

A full-length cDNA encoding an acetylcholinesterase (AChE) from Hydra magnipapillata was isolated. All of the important aromatic residues that line a catalytic gorge in cholinesterases of other species were conserved, but the sequences of peripheral anionic and choline binding sites were not. Hydra AChE, expressed in Xenopus oocytes, showed AChE activity. The gene was expressed in both ectodermal and endodermal epithelial cells except for the tentacles and basal disk. AChE gene expression was not detected in the regenerating tips in either the head or the foot, indicating that regeneration is controlled by the non-neuronal cholinergic system in Hydra.

Keywords: Cnidarian, Acetylcholinesterase, Acetylcholine, Non-neuronal cholinergic system, Hydra magnipapillata

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PII: S0014-5793(09)00998-3

doi:10.1016/j.febslet.2009.11.081

FEBS Letters
Volume 584, Issue 3 , Pages 511-516, 5 February 2010

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