| | The yeast aquaglyceroporin Fps1p is a bidirectional arsenite channelEdited by Francesc Posas Received 23 September 2009; received in revised form 12 December 2009; accepted 17 December 2009. published online 22 December 2009. Abstract The stress-activated kinase Hog1p mediates arsenic tolerance by decreasing arsenite influx through the aquaglyceroporin Fps1p in Saccharomyces cerevisiae. Unexpectedly, we found that overexpression of FPS1 increased arsenite tolerance suggesting a physiological role of Fps1p in arsenic detoxification. Consistently, during arsenite treatment transcription of FPS1 gene was strongly upregulated, while Fps1p was not degraded and remained localized to the plasma membrane. Moreover, deletion of FPS1 gene resulted in arsenate sensitivity. Finally, transport experiments revealed that Fps1p in concert with the arsenite transporter Acr3p mediates arsenite efflux. a Institute of Genetics and Microbiology, University of Wroclaw, 51-148 Wroclaw, Poland b Institute of Plant Biology, University of Wroclaw, 50-328 Wroclaw, Poland  Corresponding author. Address: Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland. Fax: +48 71 3252151.
PII: S0014-5793(09)01073-4 doi:10.1016/j.febslet.2009.12.027 © 2009 Federation of European Biochemical Societies | |
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ABSTRACT
