Properties of the intracellular transient receptor potential (TRP) channel in yeast, Yvc1

Abstract 

Transient receptor potential (TRP) channels are found among mammals, flies, worms, ciliates, Chlamydomonas, and yeast but are absent in plants. These channels are believed to be tetramers of proteins containing six transmembrane domains (TMs). Their primary structures are diverse with sequence similarities only in some short amino acid sequence motifs mainly within sequences covering TM5, TM6, and adjacent domains. In the yeast genome, there is one gene encoding a TRP-like sequence. This protein forms an ion channel in the vacuolar membrane and is therefore called Yvc1 for yeast vacuolar conductance 1. In the following we summarize its prominent features.

Abbreviations: DIDS, 4,4-diisothiocyanato-stilbene-2,2-disulfonic acid, DTT, dithiothreitol, GFP, green fluorescent protein, GOF, gain-of-function, InsP₃, inositol 1,4,5-triphosphate, Pmc1, vacuolar Ca²⁺-ATPase, TM, transmembrane domain, TRP, transient receptor potential, Vcx1, vacuolar Ca²⁺/H⁺ exchanger, V_h, holding potential, Yvc1, yeast vacuolar conductance 1

Keywords: Yeast vacuolar conductance 1, Yeast cation channel, Tonoplast recording, Transient receptor potential channel, Calcium release