Use of a redox-sensing GFP (c-roGFP1) for real-time monitoring of cytosol redox status in Arabidopsis thaliana water-stressed plants

Abstract 

Using Arabidopsis plants transformed with a redox-sensing green fluorescent protein targeted to the cytosol (c-roGFP1), we have demonstrated, in real time, measurements of reversible changes of redox status in the cytosol of plants subjected to a gradual water-stress, followed by re-watering. Plants sensed water stress, and changed the redox potential of their cytosol to a more oxidized value after a gradually-imposed water stress. Small variations in the cytosol redox potential and ascorbate (AA) values suggest that this parameter was tightly regulated. The re-watering was paralleled by a return of water stress, redox potential and ascorbate to initial values, showing the reversibility of water stress and its consequences.

Abbreviations: AA, ascorbate, c-roGFP, cytosol-redox-sensing green fluorescent protein, ROS, reactive oxygen species

Keywords: Ascorbate, c-roGFP1, Cytosol, Recovery, Redox status, Water stress, Arabidopsis thaliana