FEBS Letters
Volume 584, Issue 5 , Pages 898-902, 5 March 2010

In vitro regulation of circadian phosphorylation rhythm of cyanobacterial clock protein KaiC by KaiA and KaiB

Edited by Jesus Avila

  • Masato Nakajima

      Affiliations

    • These authors contributed equally to this work.
    • Present address: Laboratory for Systems Biology, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan.
    • ,
  • Hiroshi Ito

      Affiliations

    • These authors contributed equally to this work.
    • Present address: Division of Advanced Sciences, Ochadai Academic Production, Ochanomizu University, 2-1-1 Ohtsuka, Bunkyo-ku, Tokyo 112-8610, Japan.
    • ,
  • Takao Kondo

      Affiliations

    • Corresponding Author InformationCorresponding author. Fax: +81 52 789 2963.

Division of Biological Science, Graduate School of Science, Nagoya University and CREST, Japan Science and Technology Agency (JST), Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan

Received 25 September 2009; received in revised form 29 December 2009; accepted 8 January 2010. published online 14 January 2010.

Abstract 

Biochemical circadian oscillation of KaiC phosphorylation, by mixing three Kai proteins and ATP, has been proven to be the central oscillator of the cyanobacterial circadian clock. In vivo, the intracellular levels of KaiB and KaiC oscillate in a circadian fashion. By scrutinizing KaiC phosphorylation rhythm in a wide range of Kai protein concentrations, KaiA and KaiB were found to be “parameter-tuning” and “state-switching” regulators of KaiC phosphorylation rhythm, respectively. Our results also suggest a possible entrainment mechanism of the cellular circadian clock with the circadian variation of intracellular levels of Kai proteins.

Keywords: Circadian clock, Cyanobacterium, KaiC phosphorylation rhythm, Entrainment

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PII: S0014-5793(10)00035-9

doi:10.1016/j.febslet.2010.01.016

FEBS Letters
Volume 584, Issue 5 , Pages 898-902, 5 March 2010

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