FEBS Letters
Volume 584, Issue 5 , Pages 903-910, 5 March 2010

Identification of a novel splicing isoform of murine CGI-58

Edited by Robert Barouki

  • Xingyuan Yang
    • ,
  • Xin Lu
    • ,
  • Jun Liu

      Affiliations

    • Corresponding Author InformationCorresponding author. Address: Department of Pediatrics, University of Kentucky, BBSRB B357, 741 South Limestone Street, Lexington, KY 40536-0509, USA. Fax: +1 859 257 2120.

Department of Pediatrics, University of Kentucky, Lexington, KY, USA

Kentucky Pediatric Research Institute, University of Kentucky, Lexington, KY, USA

Received 22 October 2009; received in revised form 13 December 2009; accepted 23 December 2009. published online 18 January 2010.

Abstract 

The comparative gene identification-58 (CGI-58) gene, mutations of which are linked to Chanarin-Dorfman syndrome, encodes a protein of the α/β hydrolase domain subfamily. We report here a new alternative splicing isoform of the murine CGI-58 gene, termed mCGI-58S. Sequence comparison indicates the lack of second and third exons in this cDNA variant. While the full-length protein displayed perilipin-dependent localization to lipid droplets, mCGI-58S showed a predominant cytoplasmic staining when expressed in cells. mCGI-58S was incapable of activating adipose triglyceride lipase but retained the capacity to acylate lysophosphatidic acid. Overexpression of mCGI-58S failed to promote lipid droplet turnover and loss of intracellular triacylglycerols. These results suggest that this splicing event may be involved in the regulation of lipid homeostasis.

Abbreviations: CGI-58, comparative gene identification-58, CDS, Chanarin-Dorfman syndrome, LPA, lysophosphatidic acid, PA, phosphatidic acid, ATGL, adipose triglyceride lipase, TAG, triacylglycerol

Keywords: Neutral lipid storage disease, Lipolysis, Triacylglycerol, Lipid droplet, Lysophosphatidic acid

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PII: S0014-5793(10)00044-X

doi:10.1016/j.febslet.2009.12.058

FEBS Letters
Volume 584, Issue 5 , Pages 903-910, 5 March 2010

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