| | Salmonella regulation of intestinal stem cells through the Wnt/β-catenin pathwayEdited by Lukas Huber Received 16 November 2009; received in revised form 7 January 2010; accepted 14 January 2010. published online 18 January 2010. Abstract Recent studies have revealed that bacteria target stem cells for long-term survival in a Drosophila model. However, in mammalian models, little is known about bacterial infection and intestinal stem cells. Our study aims at understanding bacterial regulation of the intestinal stem cell in a Salmonella colitis mouse model. We found that Salmonella activates the Wnt/β-catenin signaling pathway that is known to regulate stem cells. We identified Salmonella protein AvrA that modulates Wnt signaling including upregulating Wnt expression, modifying β-catenin, increasing total β-catenin expression, and activating Wnt/β-catenin transcriptional activity in the intestinal epithelial cells. The numbers of stem cells and proliferative cells increased in the intestine infected with Salmonella expressing AvrA. Our study provides insights into bacterial infection and stem cell maintenance. a Department of Medicine, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA b Department of Microbiology and Immunology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA Corresponding author. Address: Department of Medicine, Box 646, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA. Fax: +1 585 275 8118.
PII: S0014-5793(10)00045-1 doi:10.1016/j.febslet.2010.01.024 © 2010 Federation of European Biochemical Societies | |
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