| | Characterization of hydrogen peroxide production by Duox in bronchial epithelial cells exposed to Pseudomonas aeruginosaEdited by Renee Tsolis Received 7 December 2009; received in revised form 11 January 2010; accepted 12 January 2010. published online 18 January 2010. Abstract Hydrogen peroxide production by the NADPH oxidase Duox1 occurs during activation of respiratory epithelial cells stimulated by purified bacterial ligands, such as lipopolysaccharide. Here, we characterize Duox activation using intact bacterial cells of several airway pathogens. We found that only Pseudomonas aeruginosa, not Burkholderia cepacia or Staphylococcus aureus, triggers H2O2 production in bronchial epithelial cells in a calcium-dependent but predominantly ATP-independent manner. Moreover, by comparing mutant Pseudomonas strains, we identify several virulence factors that participate in Duox activation, including the type-three secretion system. These data provide insight on Duox activation by mechanisms unique to P. aeruginosa. Abbreviations: ALI, air–liquid interface, ASL, airway surface liquid, CF, cystic fibrosis, CFTR, cystic fibrosis transmembrane conductance regulator, DPI, diphenylene iodonium, Duox, dual oxidase, H2O2, hydrogen peroxide, HRP, horse-radish peroxidase, HTBE, human tracheobronchial epithelial cells, LPS, lipopolysaccharide, Nox, NADPH oxidase, OSCN−, hypothiocyanite, RLU, relative luminescence unit, RT, reverse transcription, SCN−, thiocyanate, TLR, toll-like receptor, T3SS, type-three secretion system National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda 20852, USA  Corresponding author. Address: Laboratory of Host Defenses, NIAID, NIH. 12441 Parklawn Drive, 20852 Rockville MD, USA. Fax: +1 301 480 1731.
PII: S0014-5793(10)00046-3 doi:10.1016/j.febslet.2010.01.025 © 2010 Federation of European Biochemical Societies. Published by Elsevier BV. All rights reserved. | |
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