CBFA2T3–ZNF651, like CBFA2T3–ZNF652, functions as a transcriptional corepressor complex

Kumar, Raman; Cheney, Kelly M.; Neilsen, Paul M.; Schulz, Renèe B.; Callen, David F.

doi:10.1016/j.febslet.2010.01.047

« Previous Next »FEBS Letters
Volume 584, Issue 5 , Pages 859-864, 5 March 2010

CBFA2T3–ZNF651, like CBFA2T3–ZNF652, functions as a transcriptional corepressor complex

Edited by Ivan Sadowski

Raman Kumar
- Breast Cancer Genetics Group, Discipline of Medicine, University of Adelaide and SA Pathology, Adelaide, SA, Australia
- Corresponding author. Address: Breast Cancer Genetics Group, SA Pathology, Frome Road, Adelaide, SA 5000, Australia. Fax: +61 8 8222 3217.
Kelly M. Cheney
- Wohl Virion Centre, Division of Infection and Immunity, University College London, London, United Kingdom
Paul M. Neilsen
- Breast Cancer Genetics Group, Discipline of Medicine, University of Adelaide and SA Pathology, Adelaide, SA, Australia
Renèe B. Schulz
- Breast Cancer Genetics Group, Discipline of Medicine, University of Adelaide and SA Pathology, Adelaide, SA, Australia
David F. Callen
- Breast Cancer Genetics Group, Discipline of Medicine, University of Adelaide and SA Pathology, Adelaide, SA, Australia

Received 26 November 2009; received in revised form 13 January 2010; accepted 21 January 2010. published online 29 January 2010.

Abstract

A significant proportion of the human genome codes for transcription factors. Balanced activity of transcriptional activators and repressors is essential for normal development and differentiation. Previously we reported that a classical C₂H₂ zinc finger DNA binding protein ZNF652 functionally interacts with CBFA2T3 to repress transcription of genes containing ZNF652 consensus DNA binding sequence within the promoters of these target genes. Here we show that ZNF651 is a ZNF652 paralogue that shares a common DNA binding sequence with ZNF652 and represses target gene expression through the formation of a CBFA2T3–ZNF651 corepressor complex. It is suggested that CBFA2T3–ZNF651 and CBFA2T3–ZNF652 repressor complexes perform functionally similar roles in a tissue-specific manner.

Structured summary

MINT-7555667: CBFA2T3 (uniprotkb:O75081) physically interacts (MI:0915) with ZNF651 (uniprotkb:Q9UFB7) by anti tag co-immunoprecipitation (MI:0007)

Keywords: Repressor, ZNF651, ZNF652, CBFA2T3