Swa2p-dependent clathrin dynamics is critical for Flo11p processing and ‘Mat’ formation in the yeast Saccharomyces cerevisiae

Martineau, Céline N.; Melki, Ronald; Kabani, Mehdi

doi:10.1016/j.febslet.2010.02.008

« Previous Next »FEBS Letters
Volume 584, Issue 6 , Pages 1149-1155, 19 March 2010

Swa2p-dependent clathrin dynamics is critical for Flo11p processing and ‘Mat’ formation in the yeast Saccharomyces cerevisiae

Edited by Francesc Posas

Laboratoire d’Enzymologie et Biochimie Structurales (LEBS), CNRS, Bât. 34, Avenue de la Terrasse, 91198 Gif-sur-Yvette cedex, France

Received 21 December 2009; received in revised form 26 January 2010; accepted 2 February 2010. published online 05 February 2010.

Abstract

The yeast Saccharomyces cerevisiae is able to form complex multicellular structures called mats on low-density agar Petri plates. Mat formation strictly depends on Flo11p, a cell surface mannoprotein that mediates the adhesion of yeast cells to the agar surface. Here, we show that Swa2p, an auxilin ortholog required for clathrin-coated vesicle uncoating, is strictly required for biofilm formation. We show that the maturation and cellular levels of Flo11p are affected in Δswa2 cells, yet without compromising invasive growth. Both the TPR and J-domains of Swa2p, but not its clathrin-binding and ubiquitin-association motifs, are required for its function in Flo11p processing.

Keywords: Flo11, Ydj1, Swa2, Auxilin, Fungal biofilm, Clathrin