Identification of MyD88 as a novel target of miR-155, involved in negative regulation of Helicobacter pylori-induced inflammation

Tang, Bin; Xiao, Bin; Liu, Zhen; Li, Na; Zhu, En-Dong; Li, Bo-Sheng; Xie, Qing-Hua; Zhuang, Yuan; Zou, Quan-Ming; Mao, Xu-Hu

doi:10.1016/j.febslet.2010.02.063

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Volume 584, Issue 8 , Pages 1481-1486, 16 April 2010

Identification of MyD88 as a novel target of miR-155, involved in negative regulation of Helicobacter pylori-induced inflammation

Bin Tang
- Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China
- Emei Sanatorium of Chengdu Military Region, Emeishan, Sichuan 614205, China
- Bin Tang and Bin Xiao contributed equally to this work.
Bin Xiao
- Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China
- Bin Tang and Bin Xiao contributed equally to this work.
Zhen Liu
- Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China
Na Li
- Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China
En-Dong Zhu
- Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China
Bo-Sheng Li
- Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China
Qing-Hua Xie
- Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China
Yuan Zhuang
- Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China
Quan-Ming Zou
- Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China
- Corresponding authors. Address: Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, No. 30, Gaotanyan Street, Chongqing 400038, China. Fax: +86 023 68752316 (X.H. Mao).
Xu-Hu Mao
- Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China
- Corresponding authors. Address: Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, No. 30, Gaotanyan Street, Chongqing 400038, China. Fax: +86 023 68752316 (X.H. Mao).

Received 4 December 2009; received in revised form 6 February 2010; accepted 23 February 2010. published online 26 February 2010.

Edited by Tamas Dalmay

Abstract

MicroRNA-155 (miR-155) has been implicated as a central regulator of the immune system. We have previously reported that miR-155 negatively regulates Helicobacter pylori (H. pylori)-induced inflammation, but the molecular mechanism of miR-155 regulating the inflammation is not fully clear. Here, we identified myeloid differentiation protein 88 (MyD88) as a target gene of miR-155, and found that miR-155 decreased MyD88 expression at the protein but not the mRNA message level, suggesting that the miR-155-mediated inhibition is a post-transcriptional event. Furthermore, the overexpression of miR-155 led to significantly reduced IL-8 production induced by H. pylori infection. Thus, we have demonstrated that miR-155 can negatively regulate inflammation by targeting a key adaptor molecule MyD88 in inflammatory pathways.

Keywords: MiR-155, MyD88, Inflammation, Helicobacter pylori