FEBS Letters
Volume 584, Issue 8 , Pages 1493-1497, 16 April 2010

Evidence that the C-terminus of OprM is involved in the assembly of the VceAB-OprM efflux pump

Edited by Gianni Cesareni

  • Jiangping Bai

      Affiliations

    • Department of Microbiology and Immunology, Texas Tech University Health Science Center, TX 79430, USA
    • Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, China
    • ,
  • Lakysha Mosley

      Affiliations

    • Department of Microbiology and Immunology, Texas Tech University Health Science Center, TX 79430, USA
    • ,
  • Joe A. Fralick

      Affiliations

    • Department of Microbiology and Immunology, Texas Tech University Health Science Center, TX 79430, USA
    • Corresponding Author InformationCorresponding author. Address: Department of Microbiology and Immunology Texas Tech University Health Science Center 3601 4th street Lubbock, TX 79430.

Received 7 January 2010; received in revised form 23 February 2010; accepted 25 February 2010. published online 03 March 2010.

Abstract 

Although the architecture of tripartite multiple drug resistance (MDR) efflux pumps of Gram-negative bacteria has been well characterized, the means by which the components recognize each other and assemble into a functional pump remains obscure. In this study we present evidence that the C-terminal domain of the Pseudomonas aeruginosa OprM and the α-helical hairpin domain of Vibrio cholerae VceA play an important role in the recognition/specificity/recruitment step in the assembly of a functional, VceAB-OprM chimeric efflux pump. To our knowledge, this is the first evidence directly linking the C-terminal domain of an outer membrane efflux protein to its recruitment during the assembly of a tripartite efflux pump.

Keyword: Involvement of C-terminus of OprM in assembly of VceAB-OprM

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PII: S0014-5793(10)00170-5

doi:10.1016/j.febslet.2010.02.066

FEBS Letters
Volume 584, Issue 8 , Pages 1493-1497, 16 April 2010

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