Triad 1 induces apoptosis by p53 activation

Jung, Jin Hyuk; Lee, Sun-Mi; Bae, Seunghee; Lee, Su-Jae; Park, In-Chul; Jin, Young-Woo; Lee, Jae Ho; An, Sungkwan

doi:10.1016/j.febslet.2010.03.011

« Previous Next »FEBS Letters
Volume 584, Issue 8 , Pages 1565-1570, 16 April 2010

Triad 1 induces apoptosis by p53 activation

Edited by Varda Rotter

Jin Hyuk Jung
- Functional Genome Research Institute, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea
- These authors contributed equally to this work.
Sun-Mi Lee
- Functional Genome Research Institute, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea
- These authors contributed equally to this work.
Seunghee Bae
- Functional Genome Research Institute, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea
Su-Jae Lee
- Department of Chemistry, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea
In-Chul Park
- Laboratory of Functional Genomics, Korea Institute of Radiological and Medical Sciences, 215-4 Gongneung-dong, Nowon-gu, Seoul 139-706, Republic of Korea
Young-Woo Jin
- Division of Radiation Effect Research, Radiation Health Research Institute of KHNP, 388-1 Ssangmoon-dong, Dongbong-gu, Seoul 132-703, Republic of Korea
Jae Ho Lee
- Functional Genome Research Institute, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea
- Corresponding author. Fax: +82 2 3437 4055.
Sungkwan An
- Functional Genome Research Institute, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea
- LIFEnGENE, Inc., Venture Business Supporting Center, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea
- Corresponding author. Address: Functional Genome Research Institute, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea. Fax: +82 2 3437 4055.

Received 13 December 2009; received in revised form 2 March 2010; accepted 7 March 2010. published online 10 March 2010.

Abstract

Triad 1 (2 RING [really interesting new gene] fingers and DRIL [double RING finger linked] 1) is an E3 ligase that induces apoptosis and clonogenic inhibition in myeloid cells through Gfi-1 stabilization. Here we demonstrate that Triad 1 induces apoptosis in several cancer cell lines including MCF7, A549, U2OS, and HCT 116 p53^+/+ cells via its RING ligase activity. Interestingly, in these cancer cells, Triad 1-induced apoptosis is not mediated by Gfi-1 stabilization but is instead p53-dependent. Moreover, Triad 1 promotes transactivation of p53. These results suggest that Triad 1 can induce apoptosis through its ligase activity via p53 activation.

Abbreviations: RING, really interesting new gene, DRIL, double RING finger linked

Keywords: Triad 1, Gfi-1, Apoptosis, p53, RING finger