FEBS Letters
Volume 584, Issue 11 , Pages 2237-2241, 3 June 2010

The domain structure of talin: Residues 1815–1973 form a five-helix bundle containing a cryptic vinculin-binding site

Edited by Judit Ovádi

  • Benjamin T. Goult

      Affiliations

    • Department of Biochemistry, University of Leicester, Lancaster Road, Leicester, LE1 9HN, UK
    • ,
  • Alexandre R. Gingras

      Affiliations

    • Department of Biochemistry, University of Leicester, Lancaster Road, Leicester, LE1 9HN, UK
    • ,
  • Neil Bate

      Affiliations

    • Department of Biochemistry, University of Leicester, Lancaster Road, Leicester, LE1 9HN, UK
    • ,
  • Igor L. Barsukov

      Affiliations

    • School of Biological Sciences, University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK
    • ,
  • David R. Critchley

      Affiliations

    • Department of Biochemistry, University of Leicester, Lancaster Road, Leicester, LE1 9HN, UK
    • ,
  • Gordon C.K. Roberts

      Affiliations

    • Department of Biochemistry, University of Leicester, Lancaster Road, Leicester, LE1 9HN, UK
    • Corresponding Author InformationCorresponding author. Fax: +44 116 229 7018.

Received 3 February 2010; received in revised form 24 March 2010; accepted 8 April 2010. published online 16 April 2010.

Abstract 

Talin is a large flexible rod-shaped protein that activates the integrin family of cell adhesion molecules and couples them to cytoskeletal actin. Its rod region consists of a series of helical bundles. Here we show that residues 1815–1973 form a 5-helix bundle, with a topology unique to talin which is optimally suited for formation of a long rod such as talin. This is much more stable than the 4-helix (1843–1973) domain described earlier and as a result its vinculin binding sequence is inaccessible to vinculin at room temperature, with implications for the overall mechanism of the talin-vinculin interaction.

Structured summary

MINT-7722300, MINT-7760951: Talin-1 (uniprotkb:P26039) and Vinculin (uniprotkb:P12003) bind (MI:0407) by molecular sieving (MI:0071)

Abbreviations: HSQC, heteronuclear single quantum coherence, SCOP, structural classification of proteins, VBS, vinculin-binding site

Keywords: Talin, Vinculin, NMR, Domain structure, Helical bundle

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PII: S0014-5793(10)00298-X

doi:10.1016/j.febslet.2010.04.028

FEBS Letters
Volume 584, Issue 11 , Pages 2237-2241, 3 June 2010

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