FEBS Letters
Volume 584, Issue 13 , Pages 2791-2795, 2 July 2010

The archaeal exosome localizes to the membrane

Edited by Ulrike Kutay

  • Verena Roppelt

      Affiliations

    • Institut für Mikrobiologie und Molekularbiologie der JLU Gießen, Heinrich-Buff-Ring 26-32, D-35392 Gießen, Germany
    • ,
  • Cédric F.V. Hobel

      Affiliations

    • Max-Planck-Institut für Entwicklungsbiologie, Spemannstr. 35, D-72076 Tübingen, Germany
    • ,
  • Sonja V. Albers

      Affiliations

    • Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch Straße, D-35043 Marburg, Germany
    • ,
  • Christian Lassek

      Affiliations

    • Institut für Mikrobiologie und Molekularbiologie der JLU Gießen, Heinrich-Buff-Ring 26-32, D-35392 Gießen, Germany
    • ,
  • Heinz Schwarz

      Affiliations

    • Max-Planck-Institut für Entwicklungsbiologie, Spemannstr. 35, D-72076 Tübingen, Germany
    • ,
  • Gabriele Klug

      Affiliations

    • Institut für Mikrobiologie und Molekularbiologie der JLU Gießen, Heinrich-Buff-Ring 26-32, D-35392 Gießen, Germany
    • ,
  • Elena Evguenieva-Hackenberg

      Affiliations

    • Institut für Mikrobiologie und Molekularbiologie der JLU Gießen, Heinrich-Buff-Ring 26-32, D-35392 Gießen, Germany
    • Corresponding Author InformationCorresponding author. Fax: +49 641 9933549.

Received 14 January 2010; received in revised form 22 April 2010; accepted 4 May 2010. published online 17 May 2010.

Abstract 

We studied the cellular localization of the archaeal exosome, an RNA-processing protein complex containing orthologs of the eukaryotic proteins Rrp41, Rrp42, Rrp4 and Csl4, and an archaea-specific subunit annotated as DnaG. Fractionation of cell-free extracts of Sulfolobus solfataricus in sucrose density gradients revealed that DnaG and the active-site comprising subunit Rrp41 are enriched together with surface layer proteins in a yellow colored ring, implicating that the exosome is membrane-bound. In accordance with this assumption, DnaG and Rrp41 were detected at the periphery of the cell by immunofluorescence microscopy. Our finding suggests that RNA processing in Archaea is spatially organized.

Structured summary

MINT-7891213: Rrp41 (uniprotkb:Q9UXC2) and DnaG (uniprotkb:P95980) colocalize (MI:0403) by cosedimentation in solution (MI:0028)

MINT-7891235: Rrp41 (uniprotkb:Q9UXC2), DnaG (uniprotkb:P95980) and SlaA (uniprotkb:Q2M1E7) colocalize (MI:0403) by cosedimentation through density gradient (MI:0029)

MINT-7891278: Rrp41 (uniprotkb:Q9UXC2) and DnaG (uniprotkb:P95980) colocalize (MI:0403) by fluorescence microscopy (MI:0416)

Keywords: Archaea, Localization, Membrane, Exosome, Sulfolobus

Abbreviation: rRNA, ribosomal RNA

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PII: S0014-5793(10)00406-0

doi:10.1016/j.febslet.2010.05.013

FEBS Letters
Volume 584, Issue 13 , Pages 2791-2795, 2 July 2010

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