The nucleoporin Nup153 maintains nuclear envelope architecture and is required for cell migration in tumor cells

Abstract 

Nucleoporin 153 (Nup153), a component of the nuclear pore complex (NPC), has been implicated in the interaction of the NPC with the nuclear lamina. Here we show that depletion of Nup153 by RNAi results in alteration of the organization of the nuclear lamina and the nuclear lamin-binding protein Sun1. More striking, Nup153 depletion induces a dramatic cytoskeletal rearrangement that impairs cell migration in human breast carcinoma cells. Our results point to a very prominent role of Nup153 in connection to cell motility that could be exploited in order to develop novel anti-cancer therapy.

Structured summary

MINT-7893777: Lamin-A/C (uniprotkb:P02545) and NUP153 (uniprotkb:P49790) colocalize (MI:0403) by fluorescence microscopy (MI:0416)

MINT-7893761: sun1 (uniprotkb:Q9D666) and Lamin-A/C (uniprotkb:P02545) colocalize (MI:0403) by fluorescence microscopy (MI:0416)

Keywords: Nucleoporin, Nucleoporin 153, Nuclear pore complex, Cell migration, Nuclear lamina, Cytoskeleton

Abbreviations: cNLS, classical nuclear localization sequence, EdU, ethynyl deoxyuridine, LINC, linker of nucleoskeleton and cytoskeleton, MTOC, microtubule-organizing centre, NE, nuclear envelope, NPC, nuclear pore complex, Nup153, nucleoporin 153