FEBS Letters
Volume 584, Issue 16 , Pages 3601-3608, 20 August 2010

Galectin-3 secreted by human umbilical cord blood-derived mesenchymal stem cells reduces amyloid-β42 neurotoxicity in vitro

Edited by Jesus Avila

  • Ju-Yeon Kim

      Affiliations

    • Biomedical Research Institute, MEDIPOST Co., Ltd., Seoul 137-874, Republic of Korea
    • Department of Physiology, College of Medicine, The Catholic University of Korea, Republic of Korea
    • ,
  • Dong Hyun Kim

      Affiliations

    • Biomedical Research Institute, MEDIPOST Co., Ltd., Seoul 137-874, Republic of Korea
    • ,
  • Dal-Soo Kim

      Affiliations

    • Biomedical Research Institute, MEDIPOST Co., Ltd., Seoul 137-874, Republic of Korea
    • ,
  • Ji Hyun Kim

      Affiliations

    • Biomedical Research Institute, MEDIPOST Co., Ltd., Seoul 137-874, Republic of Korea
    • ,
  • Sang Young Jeong

      Affiliations

    • Biomedical Research Institute, MEDIPOST Co., Ltd., Seoul 137-874, Republic of Korea
    • ,
  • Hong Bae Jeon

      Affiliations

    • Biomedical Research Institute, MEDIPOST Co., Ltd., Seoul 137-874, Republic of Korea
    • ,
  • Eun Hui Lee

      Affiliations

    • Department of Physiology, College of Medicine, The Catholic University of Korea, Republic of Korea
    • ,
  • Yoon Sun Yang

      Affiliations

    • Biomedical Research Institute, MEDIPOST Co., Ltd., Seoul 137-874, Republic of Korea
    • ,
  • Wonil Oh

      Affiliations

    • Biomedical Research Institute, MEDIPOST Co., Ltd., Seoul 137-874, Republic of Korea
    • ,
  • Jong Wook Chang

      Affiliations

    • Biomedical Research Institute, MEDIPOST Co., Ltd., Seoul 137-874, Republic of Korea
    • Corresponding Author InformationCorresponding author. Fax: +82 2 475 1991.

Received 20 April 2010; received in revised form 13 July 2010; accepted 16 July 2010. published online 22 July 2010.

Abstract 

In this study, we found that expression and secretion of galectin-3 (GAL-3) were upregulated by amyloid-β42 (Aβ42) exposure in human umbilical cord blood-derived mesenchymal stem cell (hUCB-MSC) without cell death. Aβ42-exposed rat primary cortical neuronal cells co-treated with recombinant GAL-3 were protected from neuronal death in a dose-dependent manner. hUCB-MSCs were cocultured with Aβ42-exposed rat primary neuronal cells or the neuroblastoma cell line, SH-SY5Y in a Transwell chamber. Coculture of hUCB-MSCs reduced cell death of Aβ42-exposed neurons and SH-SY5Y cells. This neuroprotective effect of hUCB-MSCs was reduced significantly by GAL-3 siRNA. These data suggested that hUCB-MSC-derived GAL-3 is a survival factor against Aβ42 neurotoxicity.

Keywords: hUCB-MSC, Amyloid-β, Galectin-3, Neuroprotection

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PII: S0014-5793(10)00586-7

doi:10.1016/j.febslet.2010.07.028

FEBS Letters
Volume 584, Issue 16 , Pages 3601-3608, 20 August 2010

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