The oocyte-specific transcription factor, Nobox, regulates the expression of Pad6, a peptidylarginine deiminase in the oocyte

Choi, Mikyung; Lee, Ok-Hee; Jeon, Sanghyun; Park, Miseon; Lee, Dong Ryul; Ko, Jeong-Jae; Yoon, Tae Ki; Rajkovic, Aleksandar; Choi, Youngsok

doi:10.1016/j.febslet.2010.07.037

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Volume 584, Issue 16 , Pages 3629-3634, 20 August 2010

The oocyte-specific transcription factor, Nobox, regulates the expression of Pad6, a peptidylarginine deiminase in the oocyte

Edited by Angel Nebrada

Mikyung Choi
- Department of Biomedical Science, CHA University, Seoul 135-081, Republic of Korea
- These authors are equally contributed.
Ok-Hee Lee
- Severance Hospital Integrative Research Institute for Cerebral & Cardiovascular Disease, Yonsei University Health System, Seoul 120-752, Republic of Korea
- These authors are equally contributed.
Sanghyun Jeon
- Department of Biomedical Science, CHA University, Seoul 135-081, Republic of Korea
Miseon Park
- Fertility Center of Gangnam Medical Center, Department of Obstetrics and Gynecology, CHA University, Seoul 135-081, Republic of Korea
Dong Ryul Lee
- Department of Biomedical Science, CHA University, Seoul 135-081, Republic of Korea
- Fertility Center of Gangnam Medical Center, Department of Obstetrics and Gynecology, CHA University, Seoul 135-081, Republic of Korea
Jeong-Jae Ko
- Department of Biomedical Science, CHA University, Seoul 135-081, Republic of Korea
Tae Ki Yoon
- Fertility Center of Gangnam Medical Center, Department of Obstetrics and Gynecology, CHA University, Seoul 135-081, Republic of Korea
Aleksandar Rajkovic
- Magee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA 15213, USA
Youngsok Choi
- Department of Biomedical Science, CHA University, Seoul 135-081, Republic of Korea
- Fertility Center of Gangnam Medical Center, Department of Obstetrics and Gynecology, CHA University, Seoul 135-081, Republic of Korea
- Corresponding author at: Department of Biomedical Science, CHA University, Seoul 135-081, Republic of Korea. Fax: +82 2 3468 3446.

Received 4 June 2010; received in revised form 19 July 2010; accepted 20 July 2010. published online 26 July 2010.

Abstract

Nobox is an oocyte-specific transcriptional regulator. Nobox deficiency disrupts early folliculogenesis and the expression of oocyte-specific genes in mice. In the present study, we found that peptidylarginine deiminase 6 (Pad6) was downregulated in Nobox-null ovaries. Pad6 is preferentially expressed in oocytes and its transcript is detectable at embryonic day 16.5. In addition, we identified one Nobox DNA-binding element (NBE) within the mouse Pad6 promoter. The NBE includes a core sequence TAATTA. Sequence-specific binding of Nobox to the TAATTA motif was confirmed. Nobox overexpression augmented transcriptional activity of a luciferase reporter driven by mouse Pad6. Our findings indicate that Nobox is a critical regulator that orchestrates oocyte-specific genes such as Pad6 during folliculogenesis.

Keywords: Nobox, Folliculogenesis, Pad6, Transcription

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PII: S0014-5793(10)00595-8

doi:10.1016/j.febslet.2010.07.037

Refers to corrigendum:

Corrigendum to “The oocyte-specific transcription factor, Nobox, regulates the expression of Pad6, a peptidylarginine deiminase in the oocyte” [FEBS Lett. 584 (2010) 3629–3634] , 13 October 2010
Mikyung Choi, Ok-Hee Lee, Sanghyun Jeon, Miseon Park, Dong Ryul Lee, Jeong-Jae Ko, Tae Ki Yoon, Aleksandar Rajkovic, Youngsok Choi
FEBS Letters 5 November 2010 (Vol. 584, Issue 21, Page 4490)
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