FEBS Letters
Volume 584, Issue 18 , Pages 3923-3928, 24 September 2010

Identification of ptpro as a novel target gene of Wnt signaling and its potential role as a receptor for Wnt

Edited by Lukas Huber

  • Minseong Kim

      Affiliations

    • These authors contributed equally.
    • ,
  • Hanjun Kim

      Affiliations

    • These authors contributed equally.
    • ,
  • Eek-hoon Jho

      Affiliations

    • Corresponding Author InformationCorresponding author. Fax: +82 22210 2888.

Department of Life Science, The University of Seoul, 90 Jeonnong-dong, Dongdaemun-gu, Seoul 130-743, Republic of Korea

Received 4 August 2010; received in revised form 19 August 2010; accepted 21 August 2010. published online 30 August 2010.

Abstract 

Wnt/β-catenin signaling plays critical roles in embryonic development and tissue homeostasis in adults by controlling the expression of target genes. We found that expression of ptpro, which encodes a protein tyrosine phosphatase receptor type O (PTPRO), was induced by Wnt/β-catenin signaling in a T cell factor/lymphoid enhancer factor dependent manner. Biochemical assays found that PTPRO interacted with Wnt via its extracellular domain. In addition, ectopic expression of this extracellular domain inhibited Wnt-mediated reporter activity. These results suggest that ptpro is a target gene of Wnt/β-catenin signaling and that PTPRO may function as a novel receptor for Wnt.

Structured summary

MINT-7992076: Ptpro (uniprotkb:Q7TSY7) physically interacts (MI:0915) with Wnt3a (uniprotkb:P27467) by anti tag coimmunoprecipitation (MI:0007)

MINT-7992094: Ptpro (uniprotkb:Q7TSY7) physically interacts (MI:0915) with Wnt-3a (uniprotkb:P27467) by cross-linking study (MI:0030)

Keywords: Wnt, protein tyrosine phosphatase receptor type O, β-Catenin, T cell factor/lymphoid enhancer factor, Wnt receptor

Abbreviations: PTPRO, protein tyrosine phosphatase receptor type O, APC, adenomatous poly coli, GSK3β, glycogen synthase kinase 3β, Tcf/Lef1, T cell factor/lymphoid enhancer factor, LRP5/6, low density lipoprotein receptor-related protein 5/6, RT-PCR, reverse transcriptase-PCR, ChIP, chromatin immunoprecipitation, Fc, immunoglobulin Fc domain

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PII: S0014-5793(10)00696-4

doi:10.1016/j.febslet.2010.08.034

FEBS Letters
Volume 584, Issue 18 , Pages 3923-3928, 24 September 2010

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