MicroRNA-24 targeting RNA-binding protein DND1 in tongue squamous cell carcinoma

Liu, Xiqiang; Wang, Anxun; Heidbreder, Caroline E.; Jiang, Lu; Yu, Jinsheng; Kolokythas, Antonia; Huang, Lei; Dai, Yang; Zhou, Xiaofeng

doi:10.1016/j.febslet.2010.08.040

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Volume 584, Issue 18 , Pages 4115-4120, 24 September 2010

MicroRNA-24 targeting RNA-binding protein DND1 in tongue squamous cell carcinoma

Edited by Tamas Dalmay

Xiqiang Liu
- Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
Anxun Wang
- Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
- Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China
Caroline E. Heidbreder
- Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
Lu Jiang
- Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
Jinsheng Yu
- Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
Antonia Kolokythas
- Department of Oral and Maxillofacial Surgery, College of Dentistry, Multidisciplinary Head and Neck Oncology Program, University of Illinois at Chicago, Chicago, IL, USA
Lei Huang
- Department of Bioengineering, College of Engineering, University of Illinois at Chicago, Chicago, IL, USA
Yang Dai
- Department of Bioengineering, College of Engineering, University of Illinois at Chicago, Chicago, IL, USA
Xiaofeng Zhou
- Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
- Graduate College, UIC Cancer Center, University of Illinois at Chicago, Chicago, IL, USA
- Corresponding author. Address: University of Illinois at Chicago, College of Dentistry, 801 S. Paulina Street, Room 530C, Chicago, IL 60612, USA. Fax: +1 312 413 1604.

Received 3 August 2010; received in revised form 26 August 2010; accepted 27 August 2010. published online 02 September 2010.

Abstract

Deregulations of microRNA have been frequently observed in tongue squamous cell carcinoma (TSCC), but their roles in tumorigenesis are not entirely clear. Here, we reported the up-regulation of miR-24 in TSCC. MiR-24 up-regulation reduced the expression of RNA-binding protein dead end 1 (DND1). Knockdown of miR-24 led to enhanced expression of DND1. The direct targeting of miR-24 to the DND1 mRNA was predicted bioinformatically and confirmed by luciferase reporter gene assays. Furthermore, the miR-24-mediated change in DND1 expression suppressed the expression of cyclin-dependent kinase inhibitor 1B (CDKN1B), and also led to enhanced proliferation and reduced apoptosis in TSCC cells.

Keywords: MicroRNA, miR-24, Dead end 1, Cyclin-dependent kinase inhibitor 1B, Proliferation, Tongue squamous cell carcinoma